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Creators/Authors contains: "Yang, Qin"

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  1. Summary

    Southern leaf blight (SLB), caused by the necrotrophic fungal pathogenCochliobolus heterostrophus(anamorphBipolaris maydis), is a major foliar disease which causes significant yield losses in maize worldwide. A major quantitative trait locus,qSLB3.04, conferring recessive resistance to SLB was previously mapped on maize chromosome 3.

    Using a combination of map‐based cloning, association analysis, ethyl methanesulfonate and transposon mutagenesis, and CRISPR‐Cas9 editing, we demonstrate that a leucine‐rich repeat receptor‐like kinase gene which we have calledChSK1(Cochliobolus heterostrophus Susceptibility Kinase 1) atqSLB3.04causes increased susceptibility to SLB. Genes of this type have generally been associated with the defense response.

    We present evidence thatChSK1may be associated with suppression of the basal immune response.

    These findings contribute to our understanding of plant disease susceptibility genes and the potential to use them for engineering durable disease resistance.

     
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  2. Abstract

    Organic–inorganic hybrid perovskites (OIHPs) have been explosively investigated mainly due to their potential applications in optoelectronics. Despite the electronic charge transport, phenomena regarding the spin‐polarized electronic transport in OIHPs‐based spintronic devices and the role of ferromagnet/OIHP spinterfaces remain unclear. In this work, the spin injection, accumulation, transport, and detection at room temperature for a vertical perovskite spin valve (PeSV) consisting of Ni/CH3NH3PbI3−xClx/Ni is reported. An in‐plane anisotropic magnetoresistance (AMR) and a PeSV related magnetoresistance (MR) show remarkable magnetic switching behaviors due to the formation of Ni/CH3NH3PbI3−xClxspinterfaces, and the ferromagnetic coupling between two spin quantization axes of the spinterfaces. With assists of capacitance–frequency (C− −f) measurements under magnetic fields, the spin accumulation that occurs at the Ni/CH3NH3PbI3−xClxinterface can be detected at the spin parallel (↑↑) and antiparallel (↑↓) configurations. Owing to a strong orbital interaction at the Ni/CH3NH3PbI3−xClxhybrid interface, the spin‐sensitive electron paramagnetic spectroscopy (EPR) reveals significant change of the magnetic moment (µ). It is believed that the solution processed CH3NH3PbI3−xClxand the formation of the Ni/CH3NH3PbI3−xClxspinterface may hold an exceptionally important role for future hybrid optospintronic applications.

     
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  3. SUMMARY

    Genome‐wide association (GWA) studies can identify quantitative trait loci (QTL) putatively underlying traits of interest, and nested association mapping (NAM) can further assess allelic series. Near‐isogenic lines (NILs) can be used to characterize, dissect and validate QTL, but the development of NILs is costly. Previous studies have utilized limited numbers of NILs and introgression donors. We characterized a panel of 1270 maize NILs derived from crosses between 18 diverse inbred lines and the recurrent inbred parent B73, referred to as the nested NILs (nNILs). The nNILs were phenotyped for flowering time, height and resistance to three foliar diseases, and genotyped with genotyping‐by‐sequencing. Across traits, broad‐sense heritability (0.4–0.8) was relatively high. The 896 genotyped nNILs contain 2638 introgressions, which span the entire genome with substantial overlap within and among allele donors. GWA with the whole panel identified 29 QTL for height and disease resistance with allelic variation across donors. To date, this is the largest and most diverse publicly available panel of maize NILs to be phenotypically and genotypically characterized. The nNILs are a valuable resource for the maize community, providing an extensive collection of introgressions from the founders of the maize NAM population in a B73 background combined with data on six agronomically important traits and from genotyping‐by‐sequencing. We demonstrate that the nNILs can be used for QTL mapping and allelic testing. The majority of nNILs had four or fewer introgressions, and could readily be used for future fine mapping studies.

     
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